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Objective:To investigate the effect of Naoxintong capsule combined with butylphthalide injection on inflammatory factors, oxidative stress response and hemorheology in patients with acute cerebral infarction. Methods:Eighty-six patients with acute cerebral infarction who received treatment in Zhuji Hospital of Traditional Chinese Medicine from December 2017 to December 2019 were included in this study. Thrombolysis and thrombectomy were contraindicated in these patients. They were randomly assigned to receive treatment either with butylphthalide injection (control group, n = 43) or butylphthalide injection and Naoxintong capsule (observation group, n = 43) for 2 weeks. Therapeutic effects, Barthel Index, inflammatory factors (C-reactive protein, intercellular adhesion molecule-1 and interleukin-6), oxidative stress response (malondialdehyde and superoxide dismutase) and hemorheology (whole blood viscosity at high and low shear rates, plasma viscosity and fibrinogen) were compared between the two groups. Results:Total effective rate in the observation group was significantly higher than that in the control group (93.02% vs. 72.09%, χ2 = 6.541, P < 0.05). After treatment, Barthel Index in the observation group was significantly higher than that in the control group [(61.51 ± 5.24) points vs. (50.43 ± 4.81) points, t = 10.215, P < 0.05). After treatment, serum levels of C-reactive protein, intercellular adhesion molecule-1, and interleukin-6 in the observation group were (4.42 ± 1.03) mg/L, (84.23 ± 5.05) μg/L and (94.33 ± 10.22) μg/L, respectively, which were significantly lower than those in the control group [(8.32 ± 1.71) mg/L, (103.51 ± 6.35) μg/L, (118.92 ± 13.31) μg/L, t = 12.810, 15.583, 9.609, all P < 0.05]. After treatment, serum malondialdehyde level in the observation group was significantly lower than that in the control group [(3.76 ± 0.78) μmol/L vs. (4.94 ± 0.90) μmol/L, t = 6.497, P < 0.05]. Serum superoxide dismutase level in the observation group was significantly higher than that in the control group [(35.76 ± 2.65) U/L vs. (30.34 ± 2.11) U/L, t = 10.492, P < 0.05]. After treatment, whole blood viscosity at high and low shear rates, plasma viscosity and fibrinogen levels in the observation group were (4.10 ± 0.51) mPa · s, (9.31 ± 1.36) mPa · s, (1.24 ± 0.26) mPa · s and (2.71 ± 0.40) g/L respectively, which were significantly lower than those in the control group [(5.72 ± 0.76) mPa · s, (11.49 ± 1.59) mPa · s, (2.21 ± 0.32) mPa · s and (3.92 ± 0.54) g/L, t = 11.607, 6.832, 15.427 11.807, all P < 0.05). Conclusion:Naoxintong capsule combined with butylphthalide injection is highly effective in the treatment of acute cerebral infarction. It can reduce inflammatory reaction and improve oxidative stress response and hemorheological changes.
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OBJECTIVE：To study the effects of the main active components of Naoxintong capsule （NXTC）on the proliferation of human umbilical vein endothelial cell（HUVEC） and its key protein JAK/STAT signal pathway ， vasoactive substances ，adhesion molecules and inflammatory factors so as to clarify the m echanism of NXTC for promoting blood circulation and removing blood stasis. METHODS ：The effects of different concentration of 12 active components [caffeic acid（1.56-200 μmol/L），ferulic acid （1.56-200 μmol/L），senkyunolide H （3.125-200 μmol/L），n-butylidenephthalide（3.125-200 μmol/L），ligustilide（1.56-200 μmol/L），cryptotanshinone（0.625-80 μmol/L），tanshinol sodium （1.56-200 μmol/L），paeoniflorin （1.56-200 μmol/L），formononetin（1.56-200 μmol/L），salvianolic acid B （1.56-200 μmol/L），catechin（1.56-200 μmol/L）and astragaloside Ⅳ（1.56-200 μmol/L）] on the proliferation of HUVECs were evaluated by CCK- 8 assay. The effects of above active components（3 dose groups ，setting up 0 μmol/L blank control group，hereinafter）on mRNA expression of key proteins JAK 2， STAT3，Akt，ERK in JAK/STAT signal pathway were measured by RT-PCR. The effects of each active component on the expression of PAI- 1，VCAM-1，ICAM-1，VEGF and NF-κB p65 were detected by ELISA. RESULTS ：Ferulic acid （6.25，25-200 μg/mL），senkyunolide H （6.25-200 μmol/L），ligustilide（200 μmol/L），cryptotanshinone（10-80 μmol/L），paeoniflorin（1.56， 6.25，12.5 μmol/L），salvianolic acid B （1.56-12.5 μmol/L，200 μmol/L）and catechin （25 μmol/L）could significantly inhibit the proliferation of HUVECs ；caffeic acid （1.56，12.5 μmol/L），ligustilide（50 μmol/L），trashinol sodium （6.25 μmol/L）and paeoniflorin（1.56，100，200 μmol/L）could significantly promote the proliferation of HUVECs （P＜0.05 or P＜0.01）. Compared with blank control group ，mRNA expression of JAK 2，STAT3 and Akt were decreased significantly in some dose groups of ferulic acid，formononetin，salvianolic acid B and astragaloside Ⅳ（P＜0.05 or P＜0.01）；the expression of PAI- 1 were significantly decreased in some dose groups of caffeic acid ，ferulic acid and n-butylphthalide；the expression of ICAM- 1 and VCAM- 1 were decreased significantly in some dose groups of caffeic acid ，ferulic acid ，n-butenylphthalide，cryptotanshinone，formononetin and catechin；the expression of NF-κB p65 were decreased significantly in some dose groups of ferulic acid ，n-butenylphthalide， formononetin，salvianolic acid B and astragaloside Ⅳ；the expression of VEGF were increased significantly in some dose groups of caffeic acid and catechin （P＜0.05 or P＜0.01）. CONCLUSIONS ：The active components of Naoxintong capsule may play the role of promoting blood circulation and removing blood stasis by inhibiting the expression of JAK/STAT signal pathway key protein mRNA and PAI- 1，ICAM-1，VCAM-1，NF-κB p65 in HUVEC ，and promoting the expression of VEGF.
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OBJECTIVE@#To investigate the synergistic effect of Naoxintong Capsule (NXTC, ) and Guhong Injection (GHI, ) on cerebral ischemia-reperfusion (I/R) injury.@*METHODS@#Forty-eight Sprague-Dawley rats were divided into 6 groups: control group, oxygen and glucose deprivation (OGD) group, nimodipine group (9.375 mg/kg), NXTC group (0.5 g/kg), GHI group (5 mL/kg) and NXTC+GHI group (0.5 g/kg NXTC+5 mL/kg GHI), after the onset of reperfusion and once per day for the following 7 days. Blood was collected 1 h after final administration, and the sera were collected. Cultured primary rat brain microvascular endothelial cells (rBMECs) were subjected to OGD to establish a cell injury model. Untreated rBMECs were used as blank control. The cell counting kit-8 assay was used to assess cell viability using the sera. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assessed using an enzyme-linked immunosorbent assay. Apoptosis was evaluated after Hoechst33342 staining using fluorescence microscopy and flow cytometry. JC-1 staining was performed to assess changes in mitochondrial membrane potential.@*RESULTS@#Statistical analysis indicated that more than 95% of the cells were rBMECs. Compared with the OGD group, the cellular morphology of the all drug delivery groups improved. In particular, the combined drug group had the most significant effect. Compared with the OGD group, all drug intervention groups induced a decrease in the apoptotic rate of rBMECs, increased the SOD levels, and decreased the MDA levels (all P<0.01). Compared with the mono-therapy groups, the NXTC+GHI group exhibited a significant improvement in the number of apoptotic rBMECs (P<0.01). All drug intervention groups showed different degrees of increase in membrane potential, and the NXTC+GHI group was higher than the NXTC or GHI group (P<0.01).@*CONCLUSION@#The combinationa application of NXTC and GHI on cerebral I/R injury clearly resulted in protective benefits.
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Animals , Rats , Apoptosis , Brain , Brain Ischemia/drug therapy , Drugs, Chinese Herbal , Endothelial Cells , Glutamine/analogs & derivatives , Plant Extracts , Rats, Sprague-Dawley , Reperfusion Injury/drug therapyABSTRACT
@#An HPLC-DAD wavelength switching method(240 nm, 280 nm, 316 nm, 403 nm)was developed for simultaneous determination of seven index components: hydroxysafflor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, kaempferol, formononetin and tanshinone IIA in Naoxintong capsule. The qualities of different batches of Naoxintong capsules were evaluated by statistical analysis. Seven index components in 20 batches of Naoxintong capsules were simultaneously determined by HPLC wavelength switching method with Capcell PAK C18 MG II column(250 mm × 4. 6 mm, 5. 0 μm). The mobile phase consisted of methanol-acetonitrile(25 ∶75, A)-0. 1% formic acid aqueous solution(B)with a gradient elution program and a flow rate of 1. 0 mL/min, and the column temperature was 30 °C. The results were analyzed by statistical analysis to evaluate the differences in the quality of Naoxintong capsules. Results showed that the seven active components were well separated and showed good linearity hydroxysafflor yellow A(403 nm)2. 30- 11. 50 mg/L(r=0. 999 2), paeoniflorin(240 nm)8. 81- 44. 05 mg/L(r=0. 999 6), ferulic acid(316 nm)1. 22- 6. 10 mg/L(r=0. 999 6), salvianolic acid B(280 nm)11. 61- 58. 05 mg/L(r=0. 999 4), kaempferol(403 nm)1. 16-5. 80 mg/L(r=0. 999 4), formononetin(240 nm)0. 12- 0. 60 mg/L(r=0. 999 5)and tanshinone IIA(280 nm)2. 28- 11. 40 mg/L(r=0. 999 5). The precision was good and RSD was less than 2. 0%, The repeatability was good and RSD was less than 2. 0%. The stability was good in 24 h. The average recoveries were between 97. 35%- 101. 02% and RSD was less than 2. 0%. The contents of target components in Naoxintong capsules, hydroxysafflor yellow A was 0. 213- 0. 369 mg/g, paeoniflorin was 1. 535- 3. 217 mg/g, ferulic acid was 0. 153- 0. 236 mg/g, salvianolic acid B was 2. 563- 3. 271 mg/g, kaempferol was 0. 103- 0. 181 mg/g, formononetin was 0. 022- 0. 028 mg/g, and tanshinone IIA was 0. 466- 0. 698 mg/g. HPLC wavelength change and gradient elution method was established for simultaneous determination of seven index components in Naoxintong capsule. The method is accurate, sensitive, reliable, and repeatable, and can be used for the quality control of Naoxintong capsule.
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Objective: Intestinal absorption liquid as medicine carriers, one or more kinds of traditional Chinese medicine protecting cardiac injury in Naoxintong capsule will be found through separation and combination of prescription. This study also can expand protecting mechanisms of Naoxintong capsule. Method: Naoxintong intestinal absorption liquids of single, combination and total prescription were prepared. H9c2 cell line exposed to H2O2 was established. Cell survival rate was determined with methyl thiazolyl tetrazolium(MTT). Cell apoptosis rate was examined by Annexin Ⅴ-FITC/PI staining with a flow cytometer. Aquaporin1(AQP1) expression was detected by Western blot. One or more kinds of traditional Chinese medicine in Naoxintong capsule which exerted protective effect from cardiac injury were screened through separation and combination of prescription. Result: As compared with control group, the protein expression level of AQP1 was significantly increased(PPPPPPConclusion: Naoxintong capsule acts as a protective role in myocardial injury through decreasing AQP1 expression and inhibiting cell apoptosis. Salviae Miltiorrhizae Radix et Rhizoma, Pheretima, Scorpio are important components in Naoxintong capsule.
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Objective To observe the effect of Naoxintong capsule on blood lipid and hemorheology in the treatment of type 2 diabetes mellitus patients complicated with cerebral infarction with Qi deficiency and blood stasis type.Methods From February 2015 to February 2017,96 type 2 diabetes mellitus patients complicated with cerebral infarction with Qi deficiency and blood stasis type who treated in the Traditional Chinese Medicine Hospital of Tongxiang were selected in the research.The patients were randomly divided into two groups according to the digital table,with 48 cases in each group.The control group was treated with routine treatment scheme,while the observation group was added Naoxintong capsule.The blood lipids and hemorheology data before and after treatment were compared between the two groups.Results After treatment,the levels of LDL-C,TG,TC and HDL-C in the observation group were (1.8 ± 0.3) mmol/L,(0.9 ± 0.4) mmol/L,(2.1 ± 0.4) mmol/L,(1.6 ± 0.7) mmol/L,respectively,which were lower than those in the control group [(2.2 ± 0.4) mmol/L,(1.2 ± 0.6) mmol/L,(2.3 ± 0.3)mmol/L,(1.3 ±0.4) mmol/L] (t =5.54,2.88,3.18,2.58,all P ＜0.05).The HCT,ESR,EEP,Fig in the observation group were (41.5 ± 1.3) ％,(16.5 ± 2.1) mm/h,(292.1 ± 18.3) s,(7.6 ± 0.4) g/L,respectively,which were lower than those in the control group[(45.6 ± 1.4)％,(21.1 ± 3.2)mm/h,(332.3 ± 19.2)s,(8.2 ± 0.3) g/L] (t =14.87,8.33,10.50,8.31,all P ＜ 0.05).Conclusion Naoxintong capsule is effective in the treatment of type 2 diabetes mellitus patients complicated with cerebral infarction with Qi deficiency and blood stasis type,and can effectively improve the related indicators of blood lipids and hemorhelogy and stabilize the carotid artery plaque,it is worthy of clinical application.
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Naoxintong capsule (NXTC) is an oral Chinese preparation produced by modern technology, derived from the classic preparation of Buyang Huanwu decoction which was recorded by WANG Qing-ren (Qing dynasty) in Yilingaicuo Juanxia Tanweilun. NXTC is composed of 16 herbs including insect herbs and some blood circulation herbs, with the effects of supplementing Qi and activating blood circulation, dispersing blood stasis and dredging collaterals. In clinical application, it is mainly used for stroke, cerebral infarction, vascular dementia, ischemic cerebrovascular disease, transient ischemic attack, coronary heart disease, angina pectoris, ischemic cardiomyopathy, diabetic cardiomyopathy, myocardial infarction, chronic heart failure, chronic complications of diabetes, essential hypertension, hyperlipidemia and other cardiovascular and cerebrovascular diseases, and has achieved good therapeutic effect on above diseases or their concurrent diseases. Its clinical efficacy is mainly achieved through the improvement in related links of brain protection, neuroprotection, cardioprotection, hemorheology, et al. Nearly 200 chemical constituents identified in NXTC are important pharmacological basis for its functions. At present, however, most of its pharmacological basic researches are focused on plant herbs, and the three kinds of insect herbs remain to be further studied. The researches on clinical effectiveness are more comprehensive; the safety study of long-term application in real world is ongoing by our team, and its results are yet to be published after finishing the study. Through the systematic and comprehensive combing and elaboration of the research progresses on the chemical compositions, pharmacological action and clinical application of NXTC, it can provide a reference for the in-depth study of this preparation, with a great significance for the quality control, secondary development and internationalization promotion of NXTC.
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Objective To investigate the effect of Naoxintong capsule on clinical efficacy and inflammatory factors in patients with ischemic stroke.Methods 81 patients with ischemic stroke were divided into two groups according to random number table method.40 patients in the control group were treated with treatment guidelines,and the observation group was treated with another Naoxintong capsule.The serum interleukin-10(IL-10),tumor necrosis factor-a(TNF-α),high sensitivity C reactive protein (hs-CRP) level,National Institutes of Health Stroke Scale (NIHSS) and clinical efficacy of two groups were compared.Results After treatment,the hs-CRP,TNF-α levels of the observation group were (12.36 ± 3.09) mg/L,(129.62 ± 29.27) mg/L,which were lower than those of the control group[(1 6.71 ± 4.29) mg/L,(186.52 ± 37.62) ng/L,t =8.189,5.287,all P ＜ 0.05).After treatment for 7d and 30d,the NIHSS scores of the observation group were (14.28 ± 3.24) points,(5.23 ± 1.47) points,which were lower than those of the control group[(18.76 ±4.53)points,(8.16 ±2.42)points,t =14.689,12.827,all P ＜0.05].IL-10 level of the observation group was (49.82 ± 6.59) pg/mL,which was higher than (42.57 ± 9.82) pg/mL of the control group (t =6.759,P ＜ 0.05).The total effective rate of the observation group was 92.68％,which was higher than 77.50％ of the control group (x2 =4.897,P ＜ 0.05).Conclusion Naoxintong capsule has significant therapeutic effect on ischemic stroke,can effectively improve the inflammatory reaction after cerebral infarction,promote the recovery of neurological function and it is worthy of promotion.
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Objective:To study the clinical efficacy of levosimendan combined with naoxintong capsule in the treatment of acute heart failure and effects on the serum amino terminal B type natriuretic peptide precursor (NT-proBNP),Galectin-3,endothelin 1 (ET-1),Cystatin C levels.Methods:90 patients with acute heart failure from March 2015 to June 2016 in our hospital were selected and divided into observation group (n=45) and control group (n=45) by lottery method.Patients in the control group were treated by levosimendan alone.Patients in the observation group were treated by Naoxintong capsule combined with levosimendan.The clinical effect,changes of serum NT-proBNP,galectin-3,ET-1,cystatin C levels before and after treatment and the incidence of adverse reactions were compared between two groups.Results:After treatment,the total effective rate was 93.33％ in the observation group,which was higher than that in the control group (77.78％) (P＜0.05);the left ventricular fractional shortening,left ventricular ejection fraction,stroke volume of both groups were higher than those before treatment,the blood pressure,heart rate and serum NT-proBNP,galectin-3,ET-1,cystatin C levels were lower than before treatment,and LVFS,LVEF,SV levels of the patients in the observation group were significantly higer than those of the control group (P＜0.05),the blood pressure,heart rate and serum NT-proBNP,galectin-3,ET-1,cystatin C levels were lower than those of the control group (P＜0.05).Conclusion:Levosimendan combined with Naoxintong Capsule could improve the therapeutic effect of acute heart failure with higher safety and reduce the levels of serum NT-proBNP,Galectin-3,ET-1 and Cystatin C.
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Objective:To study the effect of naoxintong capsules combined with nerve growth factor on the serum levels of interleukin-6 (IL-6),matrix metalloproteinase-9 (MMP-9) and S100B of patients with hypertensive intracerebral hemorrhage.Methods:88 patients with hypertensive intracerebral hemorrhage who were treated in our hospital from August 2015 to July 2016 were selected and randomly divided into the observation group and the control group The patients in the control group were treated with nerve growth factor,while the patients in the observation group were treated with naoxintong capsules combined with nerve growth factor.Then the clinical efficacy,GCS,GOS score,serum levels ofIL-6,MMP-9 and S100B were observed and compared between the two groups.Results:After treatment,the total effective rate in the observation group was significantly higher than that the control group (P＜0.05).After treatment,the GCS and GOS scores of the two groups were significantly higher than before,and the GCS and GOS of the observation group were higher than those of the control group (P＜0.05).After treatment,the serum levels of IL-6,MMP-9 and S100B in the two groups were significantly lower than before,and the observation group were lower than those of the control group (P＜0.05).There was adverse reactions in the two groups.Conclusion:Naoxintong capsule combined with nerve growth factor can reduce the serum levels of IL-6,MMP-9 and S100B in patients with hypertensive intracerebral hemorrhage,with obvious clinical curative effect and high safety.
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OBJECTIVE:To explore the effect of Buchang naoxintong capsule on the myocardial perfusion and platelet activation function in patients with acute myocardial infarction (AMI) after PCI. METHODS:100 AMI patients were randomly divided into control group and observation group by parallel random sampling,50 cases in each group. Control group was given conventional PCI, preoperatively chewing 300 mg aspirin+300 mg clopidogrel,postoperatively taking 100 mg/d aspirin+75 mg/d clopidogrel. Observation group was additionally treated 2-4 with Buchang naoxintong capsules(after bracket release),3 times a day,the treatment course for both groups was 7 d,observation period was 1 month. Infarct-related artery myocardial perfusion grade(TMPG)in 2 groups,platelet activation function indexes [CD62P,CD63 and monocyte platelet aggregation (MPA) positive expression rates] before and after treatment,the incidences of adverse cardiac events (MACE) and complications were observed and compared 15 min after PCI. Meanwhile,the incidence of adverse reactions was recorded. RESULTS:After treatment,the cases of TMPG perfusion grade 3 in observation group were significantly higher than control group,the incidence of MACE was significantly lower than control group, with statistical significance(P0.05). Before treatment,there was no obvious difference in CD62P,CD63,MPA positive expression rates in 2 groups;after treatment,CD62P,CD63,MPA positive expression rates in 2 group were significantly lower than before,and observation group was lower than control group,with significant differences(P<0.05). CONCLUSIONS:In PCI treatment of AMI patients,Buchang naoxintong capsule can rapidly improve platelet activation functions,TMPG perfusion,reduce the incidence of MACE,with good safety and no increase of complications.
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Endothelial progenitor cells (EPCs) are precursor cells of endothelial cells. Signal molecules produced by ischemia and hypoxia can promote mobilization of bone marrow EPCs to peripheral circulation and formation of novel blood vessels in tissues that are damaged during heart attack. Naoxintong capsule (NXT) has the functions of promoting blood circulation, removing blood stasis, promoting the circulation of qi and relieving pain. The various components in NXT have protective effects on blood vessels and can effectively improve the symptoms of ischemia. However, its effect on EPCs is not clear. To study the intervention effect of NXT on mobilization and homing of peripheral blood EPCs, green fluorescent protein (GFP) transgenic mice were used for bone marrow transplantation (BMT) and then unilateral hind limb ischemia model (UHLI) were constructed. For BMT, wild-type ICR mice were irradiated by CS137 and then injected with 4×106 bone marrow cells isolated from GFP mice. The bone marrow reconstitution of recipients was assessed by quantification of GFP bone marrow-derived cells (BMDC) from transplanted mice 4 weeks after BMT. The UHLI model was duplicated by ligating femoral artery and divided into three groups: the model group, the NXT group (model+NXT) and the positive control group (model+simvastatin). Flow cytometry was used to detect the proportion of GFP positive cells and the peripheral blood EPCs levels at 1, 3, 7, 14 days before and after surgery. Ischemic tissue of gastrocnemius muscle was excised at 3 and 7 days after operation for immunofluorescence staining to detect the number of GFP+ cells. The bone marrow chimerism was achieved at day 28 after BMT. There was no significant difference in the percentage of GFP positive cells between BMT mice and GFP transgenic mice. NXT and simvastatin could significantly increase the number of peripheral blood EPCs 1,3 days after surgery. Three and seven days after operation, the number of homing EPCs was significantly higher in NXT group and positive control group than that in model group (P<0.001). In conclusion, NXT can obviously promote the mobilization and homing of EPCs.
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Objective To explore influence of Naoxintong capsule combined with edaravone on clinical efficacy and vascular endothelial function of patients with type-2 diabetes complicated with cerebral infarction.Methods 80 cases of patients with type-2 diabetes complicated with cerebral infarction treated in Shengsi People's Hospital in Zhejiang from January 2014 to January 2016 were selected as the objects, and were divided into control group and study group randomly, forty-one cases in each group.The control group were given edaravone treatment, and the study group were added Naoxintong capsule on the basis of the control group, treatment for two weeks in the two groups.Clinical therapeutic effect of the two groups and changes of TCM syndrome score before and after treatment were compared, and scores of nerve function defect and daily life ability were evaluated, and changes of blood glucose and glycosylated hemoglobin levels were detected, and endothelin ( ET-1 ) , nitric oxide ( NO ) , thromboxane B2 ( TXB2 ) and 6-keto prostaglandin F1(6-keto-PGF1) were tested before and after treatment in the two groups.Adverse reactions were observed in the two groups in treatment. Results The total effective rate of the study group was significantly higher than that of the control group(P<0.05), and TCM syndrome scores of the two groups were significantly decreased after treatment in the two groups(P<0.05), which of the study group was significantly lower than that of the control group(P<0.05).After treatment, NIHSS score of the two groups significantly decreased(P<0.05), and ADL score significantly increased(P<0.05), and changes of the study group were significantly higher than those of the control group(P <0.05).Fasting blood glucose and glycosylated hemoglobin levels significantly decreased in the two groups(P<0.05), which of the study group were significantly lower than those of the control group ( P <0.05 ).Levels of ET-1 and TXB2 significantly decreased ( P <0.05 ) , levels of NO and 6-keto-PGF1 significantly increased ( P <0.05 ) , and changes in the study group were significantly higher than those in the control group(P<0.05).There was no significant difference in incidence of adverse reactions between the two groups.Conclusion The effect is obvious of Naoxintong capsule combined with edaravone in treratment of patients with type-2 diabetes complicated with cerebral infarction, and can improve vascular endothelial function of patients.It can be widely used in clinical.
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Objective To observe the therapeutic efficiency of naoxintong capsule on the patients with moderate obstructive sleep apnea hypopnea syndrom ( OSAHS ) of cognitive dysfunction. Methods Sixty pa?tients of moderate OSAHS were randomly divided into treatment group and control group, 30 cases in each group. Patients in the treatment group were given naoxintong capsule,three goals,three times a day,at the same time,with double level positive pressure ventilation therapy for 3 months. Patients in the control group accepted double level positive pressure ventilation therapy for 3 months. The questionnaires of Montreal Cognitive Assess? ment( MoCA) were administered to assess cognitive in all the patients before and after treatment. Results There was no statistically significant difference in term of MoCA score between the two groups before treatment( t=0. 14,P>0. 05). After treatment,the MoCA score of the treatment group was (25. 83+1. 70),of control group was (22. 70+2. 51),the difference was statistically significant(t=5. 62,P<0. 05). The visual space and execu?tive function,naming,attention,language,abstract,delayed recall and directional score in the treatment group af?ter treatment were (5. 03±0. 81) points,(2. 90±0. 31) points,(4. 73±0. 58) points,(2. 63±0. 49) points, (1. 97±0. 18) points,(3. 93±0. 98) points,(5. 03±0. 56) points respectively,compared with before treatment ((4.07±0.79) points,(2.17±0.59) points,(3.40±0.50) points,(2.03±0.49) points,(1.80±0.41) points,(2. 87±0. 73) points,(4. 03±0. 77) points),the differences were statistically significant (t=6. 55, 6. 89,13. 36,5. 29,2. 41,4. 87,6. 60;P<0. 05) . The naming,attention,language,abstract,delayed recall and di?rectional score in the control group after treatment were ( 2. 77 ± 0. 43 ) points, ( 4. 07 ± 0. 75 ) points, ( 2. 20 ±0. 55) points,(1. 87±0. 35) points,(3. 10±0. 66) points,(4. 67±0. 71) points respectively,ompared with be?fore treatment((2. 30±0. 65) points,(3. 43±0. 68) points,(2. 00±0. 70) points,(1. 73±0. 45) points,(2. 93 ±0. 58) points,(3. 93±0. 69) points),the differences were statistically significant(t=5. 04,4. 34,2. 26,2. 11, 2. 41,6. 28;P<0. 05) . MoCA score in treatment group than the control group have improved significantly in visu?al space and executive function,attention,language and delayed recall,directional ( t=4. 51,3. 80,3. 22,3. 86, 2. 23,P<0. 05) . Conclusion Naoxintong capsule can improve cognitive dysfunction in patients with moderate OSAHS.
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Naoxintong capsule has beneficial effects for activating blood circulation, dispersing blood stasis and dredging collateral. It is widely used in the treatment of coronary heart disease, angina pectoris, stroke and cardiovascular disease. However, the pharmacodynamic basis and possible mechanism of its preventive effects are not clear. In this study, 10 male and 10 female C57BL/6 mice were used, and were randomly divided into the control group (saline) and Naoxintong group. Adaptively fed for 7 days in common conditions, mice were given Naoxintong capsule or saline for 3 days via intragastric administration. Serum was collected from 6 mice in each group 1 h after the last administration. Serum proteins were prepared to do two-dimensional gel electrophoresis. Then image analysis and mass spectrometry detection were carried out to screen and identify the differentially expressed proteins and make bioinformatics analysis. It was found that 24 differentially expressed proteins between Naoxintong group and control group. Compared with the control group, 12 proteins were increased, and 12 were decreased. The proteins were involved in apoptosis signal pathway and vascular endothelial growth factor signal transduction pathway, in which vasohibin-1 is a negative feedback regulation factor in angiogenesis. Western blot showed that the expression of vasohibin-1 in Naoxintong group was reduced, which is consistent with the result in two-dimensional electrophoresis. Serum proteins expression is different between Naoxintong and control groups. The targets of these differentially expressed proteins include endothelial cells, inflammatory cells and platelets. The changes on proteins showed that Naoxintong capsule may ameliorate coronary heart disease and ischemic cerebrovascular disease, and provide potential biological markers to prevent ischemic disease.
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Objective To investigate the effect of Atorvastatin Combined Naoxintong Capsule treats to cerebral infarction and carotid atherosclerosis.Methods 134 patients with cerebral infarction and carotid atherosclerosis were diagnosed by MRI or CT scan.They were randomly divided into two groups.67 cases were given atorvastatin versus aspirin received routine treatment in the control group and 67 cases were used Naoxintong capsule in observation group on the basis of routine treatment in the observation group.The blood lipid,serum high sensitive C-reactive protein (hs-CRP) level,neural function defect score (NIHSS),carotid artery intima-media thickness (IMT) were comperred before and after treatment in two groups for treated with 1 courses (6 months).Results The blood lipid had no significant difference between two groups before and after treatment (P ＜ 0.05).The IMT,NIHSS score of high sensitivity C-reactive protein levels had no significant difference in two groups before and after treatment (P ＜ 0.05).The scores of NIHSS,IMT,high sensitive C-reactive protein level respectively (10.08 ± 1.82) ％,(1.06 ± 0.13)mm,(3.89 ± 1.41) mg/L of observation group were more decreased significantly than the control group (respectively (13.92 ±2.01)％,(1.24 ±0.15)mm,(5.02 ± 1.52) mg/L after treatment.Conclusion Atorvastatin Combined Naoxintong Capsule in treatment of cerebral infarction and carotid artery gruel kind hardening shows good therapeutic effect,it is worth for clinical use.
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Objective To investigate the changes in endothelial progenitor cells (EPCs) number in peripheral blood of patients with acute cerebral infarction and after Naoxintong treatment.Methods Sixty patients were selected as subject and randomly divided into aspirin group and aspirin (30 patients) + Naoxintong group (30 patients).Meanwhile 30 patients without cerebral infarction were served as control group The number of peripheral blood EPCs were detected by flow cytometry at different time point.NIH-NINDS stroke score was used to elevate the neurological function.Results Compared with the control group,number of peripheral blood EPCs significantly decreased in the early stage of acute cerebral infarction (P ＜ 0.05),and then gradually increased until 7th day,which was back to the normal level.There was a positive correlation between improvement of NIHSS and number of peripheral blood EPCs in acute cerebral infarction.Compared with aspirin group,the number of peripheral blood EPCs in Naoxintong group increased sigrificanfly [41.40 ±0.18/million cells vs 41.40 ±0.18/million cells] at 14th day in patients treated with aspirin and Naoxintong.Conclusion The number of peripheral blood EPCs showed a U shape dynamic change in acute cerebral infarctior.Increase the number of peripheral blood EPCs might improve prognosis in patients with acute cerebral infarction.On the basis of routine treatment of aspirin,Naoxintong plus aspirin treatment might improve the number of peripheral blood EPCs in acute cerebral infarction.
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Objective To investigate the effects of atorvastatin combined with Naoxintong capsules on serum lipid level and hish sensitivity C-reactive protein(hs-CRP)in patients with acute coronary syndrome(ACS).Methods 82 ACS patients were randomly divided into observation group(10 mg/d of atorvastatin and 4 capsules 3 times of Naoxintong daily,n=41) and control group(20 mg/d of simvastatin daily,n=41).The levels of serum lipid and hs-CRP were detected before and after treatment.Results 8 weeks after treatment,the levels of serum hpid[TC (4.21±0.87)mmol/L,TG(1.99±0.48)mmol/L,LDL-C(2.71±0.83) mmol/L] and serum hs-CRP(5.63±1.38)mg/L in observation group were significantly lower than those in control group[TC(4.89±0.91)mmol/L,TG (2.58±0.52)mmol/L,LDL-C(3.28±0.89)mmol/L],and serum level HDL-C(1.89±0.47)mmol/L was higher than that in control group(1.63±0.45)mmol/L(t=2.55-4.36,P<0.01～0.05).Conclusion Adjuvant therapy of Naoxintong could promote the levels of serum lipid and hs-CRP in patients with ACS.and offer a better therapeutic effect in these patients.
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@#Objective To investigate the protective effects of Naoxintong capsule on cerebral ischemia-reperfusion injury in rats.Methods 45 SD rats were randomly divided into 5 groups: sham operation group (n=5), ischemia-reperfusion group (n=10), small dose of Naoxintong capsule group (n=10), large dose of Naoxintong capsule group (n=10), CoQ-10 group (n=10). The the cerebral ischemia-reperfusion model was established by occluding bilateral carotid arteries of animal. The moisture content and activity of ATPase in brain tissue of model rat were observed respectively at ischemia 30 min plus reperfusion 30 min and ischemia 30 min plus reperfusion 60 min.Results The activities of Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase decreased (P<0.01), contents of water in brain tissues increased (P<0.01) in the ischemia-reperfusion group, compared with the sham operation group. The activities of Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase increased (P<0.01), contents of water in brain tissues decreased (P<0.01) in the Naoxintong capsule group, compared with the ischemia-reperfusion group.Conclusion Naoxintong capsule can protect the brain tissue from ischemia-reperfusion injury.
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Objective To study the functional mechanism of Naoxin tong Capsule (Ca psule for cerebral and heart diseases) on a cute coronary syndrome (ACS). Methods Totally 105 ACS p atients diagnosed with coro nary angiography were randomized into a treatment group (55 cases), and a control group (50 cases). The control group was treated routin ely with western medicine while the treatment group treated with Naoxintong Capsule, 2 capsules each time, 3 times a day, in addition to the routine western medicine. Before and 4 weeks after treatment, the serum hypersensitive C-react ive protein (hs-CRP) was measured. Before and six months af ter treatment, changes of carotid intima-media thickness (IMT) and carot id plaque were examined with Color Doppler Ultrasonography. Resu lts Four weeks after treatment, the hs-CRP level in the treatment grou p was significantly lowered than before treatmen t, and the effect was more significant than that of the control group (P